AFDye 546 Picolyl Azide

貨號
規(guī)格
價格
品牌
貨期
1284-1
1 mg
3580.0
ClickChemistryTools
現(xiàn)詢
1284-5
5 mg
10980.0
ClickChemistryTools
現(xiàn)詢
1284-25
25 mg
30700.0
ClickChemistryTools
現(xiàn)詢

Abs/Em Maxima: 554/570 nm

Extinction Coefficient: 110,000

Flow Cytometry Laser Line: 532 nm

Microscopy Laser Line: 543 nm or 546 nm

Spectrally Similar Dyes: Alexa Fluor? 546, Atto? 546, CF? 546

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AZDye? 546 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).

In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.

In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.

AZDye? 546 (Alexa Fluor? 546 equivalent) is water-soluble, and pH-insensitive from pH 4 to pH 10 orange-fluorescent dye with absorption and emission maxima at 554 and 570 nm, respectively. It can be used with the 488 nm and 532 nm laser lines. AZDye? 546 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates often used for generation of stable signal in imaging and flow cytometry.

AZDye? 546 dye is structural and spectrally identical Alexa Fluor? 546.

分子量
1050.39 (protonated)
分子式
N/A
CAS
N/A
溶(解)度
Water, DMSO, DMF
純度
>95% (HPLC)
外觀
Red solid
儲存環(huán)境
-20°C. Desiccate
運輸條件
Ambient temperature

1. Jiang, H., et al. (2014). Monitoring Dynamic Glycosylation in Vivo Using Supersensitive Click Chemistry. Bioconjugate Chem.,, 25, 698-706. [PubMed]

2. Uttamapinant, C., et al. (2012). Fast, Cell-Compatible Click Chemistry with Copper-Chelating Azides for Biomolecular Labeling. Angew. Chem. Int. Ed,., 51, 5852-56. [PubMed]

3. Gaebler, A.,et al. (2016). A highly sensitive protocol for microscopy of alkyne lipids and fluorescently tagged or immunostained proteins. J. Lipid. Res., 57, 1934-47. [PubMed]


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